Alternative Polyadenylation in response to temperature stress contributes to gene regulation in Populus trichocarpa

Abstract Background Genome-wide change of polyadenylation (polyA) sites (also known as alternative polyadenylation, APA) is emerging an important strategy gene regulation in response to stress plants. But little woody perennials that are persistently dealing with multiple abiotic stresses. Results Here, we performed a genome-wide profiling under heat and cold treatments Populus trichocarpa . Through comprehensive analysis polyA tail sequences, identified 25,919 polyA-site clusters (PACs), revealed 3429 3139 genes shifted stresses respectively. We found small proportion possessed APA affected the open reading frames; some shifts were commonly identified. Functional displaying tails suggested pathways related RNA metabolism linked regulate events both Interestingly, induced significantly more antisense PACs comparing control conditions. Furthermore, showed unique cis-element (AAAAAA) was predominately enriched downstream P. genes; this sequence signal only absent condition, indicating distinct mechanism responsive tolerance. Conclusions This work provides picture global patterns temperatures trees. show frequent potential stress, which associated distinctive signatures.